重组猪β-防御素1对大肠杆菌的抑制作用

doi:10.11843/j.issn.0366-6964.2017.07.019

Abstract

Abstract:

The present study aimed to evaluate the antibacterial activity and treatment effect of porcine beta-defensin 1 (pBD1) against E. coli in vitro and in sick chicken infected with E. coli. The inhibitory effects of pBD1 on three E. coli strains (O₁₄₁, SJZ1 and SJZ2) were detected through agar-gel diffusion method and micro-antibacterial assay in vitro, respectively. Then forty 11-day-old chickens were divided into 5 groups (n=8) randomly, including the defensin group (A), antibiotic group (B),defensin and antibiotic group (C), non-treatment group after infection (D) and negative control group (E).Group A, B, C and D were challenged with 1 mL E. coli O₁₄₁(1.25×10⁹ cfu·mL^-1) via the neck subcutaneous injection and oral administration respectively, and Group E was inoculated 1 mL nutrient broth with the same administration routes. While theE. coli -infected chicken showing the clinical symptoms, the group A, B, C and D were given orally pBD1 (50 μg per chicken each time), ceftriaxone sodium (50 mg per kg of body weight), pBD1 (50 μg per chicken each time) and ceftriaxone sodium (50 mg per kg of body weight), and the same volume of sterilized saline water once a day, respectively, and the chickens in each group were continuously treated for seven days. The results showed that the recombinant pBD1 displayed clearly antibacterial activity against E.coli in vitro and this antibacterial effect increased as the concentration of pBD1 rose within certain ranges. Compared with the non-treatment group, the reduction were exhibited in clinical symptoms, pathological changes, morbidity and mortality of the three treated group (A, B and C). The body weights of the group A treated with pBD1 and the group C with defensin and antibiotic were significantly higher (P <0.01) than that of the non-treated group (D) in the later period of infection. These results showed that the recombinant pBD1 has obviously inhibitory effect on E. coli, and has effective therapeutic effects on the sick chicken suffering the colibacillosis. The treatment effect of pBD1 was superior to that of ceftriaxone sodium and helpful to refreshment of the sick chicken. The application of pBD1 combined with ceftriaxone sodium demonstrated the better treatment effect. This study could provide scientific basis for effective treatment of the colibacillosis and lay a solid foundation for the research and development of new type antibacterial agent of pBD1.

CLC Number:

S859.797

GUO Hai-yong, YUAN Hong-xing, WANG Yun-xiao, XU Rui-tao, SONG Qin-ye. Inhibitory Effects of Recombinant Porcine Beta-defensin 1 onEscherichia coli[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1342-1348.

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Inhibitory Effects of Recombinant Porcine Beta-defensin 1 onEscherichia coli

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